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Phytochemistry And Antioxidant Evaluation Of Methanol Seed Extract And Vacuum Liquid Chromatography Fractions Of Parquentina Nigrescens (aflel) Bullock (perplocaceae)

 
  (Vol. 6 Number 2) by Edoge N, Uwaya J.o, Okunrobo L.o, *igbinaduwa P.o  
     
 

In recent time, interest has been drawn to the possible pharmacologic properties of the fruits and seeds of Parquetina nigrescens (Periplocaceae). The aim of this study was to evaluate the phytochemical composition and antioxidant properties of the seeds. Phytochemical screening was done according to standard methods. Antioxidant evaluation was carried out by DPPH radical scavenging and FRAP assay on the crude extract and its Vacuum Liquid Chromatography (VLC) fractions. Phytochemical screening revealed the presence of alkaloids, saponins, phenols, flavonoids and proteins. Antioxidant scavenging activity in both DPPH and FRAP assays revealed a concentration-dependent activity with the best activity in the extract. Total phenol and flavonoid were determined as 112.34 mg gallic acid equivalent/g of extract (mgGAE/g) and 25.53 mg quercetin equivalent/g of extract (mgQE/g) respectively. Correlation between the antioxidant activity and the phenolics was significant (P< 0.05). This study has reported to the best of our knowledge, for the first time, the anti-oxidant properties of the methanol seed extract of P. nigrescens which was significantly associated with the phenolic constituents in the seeds.

Keywords: Parquetina nigrescens, Seeds, Antioxidant, Methanol extract, VLC-fractions



 
     
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